IKKβ Kinase Enzyme System

Kinase Enzyme Systems for Detecting Kinase Activity
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Easily Screen and Profile IKKβ Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

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Catalog number selected: V4502

$ 545.00
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IKKβ Kinase Enzyme System
10µg
$ 545.00
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Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The IKKβ Kinase Enzyme System contains:

  • IKKβ Kinase, 10μg (Human, recombinant full-length). MW: ~105kDa.
  • IKKtide (KKKKERLLDDRHDSG-LDSMKDEE) Substrate; derived from human IkBA (amino acids 21–41).
  • Reaction Buffer, DTT.

Full-length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKKβ is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein, which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKβ is stimulated by TNF and IL1 and IKKβ forms a heterodimer that interacts with NIK. Overexpression of catalytically inactive IKKβ blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKβ activity in vitro and in vivo by binding to IKKβ to reduce ATP binding.

IKKβ NCBI Database Entry.

The IKKβ Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Specifications

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

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What's in the box?

Item Part # Size

IKKβ Kinase Enzyme System

V4502 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

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