CellTiter-Glo® Luminescent Cell Viability Assay
A Luminescent Assay to Determine the Number of Viable Cells in Culture
- A homogeneous "add-mix-measure" format reduces the number of steps
- Higher level of detection requires fewer cells per assay
- Process plates consecutively with a very stable luminescent signal
- Integrates with the MyGlo® Reagent Reader
Catalog Number:
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Size
Catalog Number: G7570
Catalog Number: G7571
Catalog Number: G7572
Catalog Number: G7573
Catalog Number: MG1010
CellTiter-Glo® Measures ATP, A Key Biomarker of Cell Health
The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing.
The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps.
The CellTiter-Glo® Luminescent Cell Viability Assay is a popular and trusted method used in a wide range of applications.
CellTiter-Glo® Assay offers 10-cell sensitivity; up to 5 logs linear range. The signal was read on a MyGlo® Reagent Reader.
"We perform knockdown experiments for noncoding RNAs on a number of different cell lines to identify novel cancer therapy targets. The CellTiter-Glo® Assay is an extremely easy-to-use reagent to address cell viability and obtain consistent and reproducible results."
Anan Quan, Research Assistant, Beth Israel Deaconess Medical Center.
"The CellTiter-Glo® Assay provides a very robust, easy-to-use reagent for high-throughput screening of small molecules for antiviral activity against viruses such as SARS-CoV and SARS-CoV-2 that cause a cytopathic effect in cell lines."
Dr. Colleen Jonsson, Director of the Regional Biocontainment Laboratory and Director of the Institute for the Study of Host-Pathogen Systems, University of Tennessee Health Science Center.
Read more about Dr. Jonsson's work to identify antiviral agents here.
Notes
Using 100μl of CellTiter-Glo® Reagent per assay in a 96-well format, Cat.# G7570 is sufficient to perform 100 assays; Cat.# G7571 and G7572, 1,000 assays; Cat.# G7573, 10,000 assays. Using 25μl of CellTiter-Glo® Reagent per assay in a 384-well format, Cat.# G7570 is sufficient to perform 400 assays; Cat.# G7571 and G7572, 4,000 assays; Cat.# G7573, 40,000 assays.
Customer Testimonials: Advantages of CellTiter-Glo
Bioluminescence Resource Center
Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755A | 1 × 1 vial |
|
CellTiter-Glo® Buffer |
G756A | 1 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755A | 10 × 1 vial |
|
CellTiter-Glo® Buffer |
G756A | 10 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755B | 1 × 1 vial |
|
CellTiter-Glo® Buffer |
G756B | 1 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
CellTiter-Glo® Substrate (lyophilized) |
G755B | 10 × 1 vial |
|
CellTiter-Glo® Buffer |
G756B | 10 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
MyGlo™ Reagent Reader
|
MG1000 | 1 × 1 each | |
CellTiter-Glo® 2.0 Reagent |
G9241 | 1 × 10ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Reconstituted CellTiter-Glo® Reagent can also be stored at 4°C for 48 hours with ~5% loss of activity or at 4°C for 4 days with ~20% loss of activity.
Resources
Articles
- Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins
- Impact of aerosols on liver xenobiotic metabolism: A comparison of two methods of exposure
- Tolerance to colibactin correlates with homologous recombination proficiency and resistance to irinotecan in colorectal cancer cells
- Comparative characterization of different molecular formats of bispecific antibodies targeting EGFR and PD-L1
- Overcoming preclinical safety obstacles to discover GDC-2394: A potent and selective NLRP3 inhibitor
- Elevated expression of miR-494-3p is associated with resistance to osimertinib in EGFR T790M-positive non-small cell lung cancer
- Insights into the modular design of kinase inhibitors and application to Abl and Axl
- Metformin attenuates rotenone-induced oxidative stress and mitochondrial damage via the AKT/Nrf2 pathway
- Endogenous retroviruses mediate transcriptional rewiring in response to oncogenic signaling in colorectal cancer
- A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar "Varnes"
- Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals
Other Resources
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