Legionella Viability qPCR Kits

cs333001-legionella-spp-legionella-pneumophila-viability-qpcr-kit-3

cs333002-legionella-pneumophila-viability-qpcr-kit-3

cs333003-viability-pcr-crosslinker-kit-3

Legionella qPCR Kits with Live/Dead Cell Differentiation

Get same-day results
Rapidly differentiate live and dead cells
Identify L. pneumophila or L. spp, L. pneumophila and L. pneum. Serogroup 1
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Legionella Viability qPCR Kits

Detect Live Legionella with Speed and Sensitivity

Reliable detection of Legionella pneumophila in water supplies is important to prevent occurrence of Legionnaires’ disease. Legionella testing is commonly performed using culture-based approaches, which often take 7–10 days. PCR-based detection is much faster, but it cannot differentiate between live and dead cells, which can possibly lead to false-positive results.

The Legionella Viability qPCR Kits combine the rapid protocols of PCR with the ability to discriminate between live and dead cells for fast, sensitive and specific Legionella pneumophila detection. We also offer a triplex kit that can detect Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1 in a single assay.

How the Legionella Viability qPCR Kit Differentiates Live/Dead Cells

The kit contains a cell-impermeable intercalating crosslinker that exclusively binds to DNA from membrane-compromised dead, dying or lysed cells. Once bound, the molecule irreversibly crosslinks DNA, making it unamplifiable in PCR reactions. This ensures that signal from the qPCR detection reaction is exclusively from viable cells and not dead cells, therefore eliminating false-positive results.

How the Legionella pneumophila PCR Kit Differentiates Live/Dead Cells---18076ma

Crosslinker treatment (10µM) greatly reduces false-positive results caused by qPCR signal from dead cells.---18077ma — Crosslinker treatment (10µM) greatly reduces false-positive results caused by qPCR signal from dead cells.

Sample to Results in 6 Hours

Legionella pneumophila Viability qPCR Kits Workflow---18078mb — Created with BioRender.com

Triplex to Detect Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1

Knowing whether infectious non-pneumophila Legionella species are present can help inform mitigation and response plans. You can conveniently detect Legionella pneumophila and members of the Legionella genus in the same qPCR reaction with the Legionella spp./Legionella pneumophila/L. pneum. Serogroup 1 viability qPCR kit.

Standard curve for detection of L. spp./L. pneumophila/L. pneum. Serogroup 1 using the Legionella Viability qPCR Kits.

Available Kits

Legionella spp./Legionella pneumophila/L. pneum. Serogroup 1 Viability qPCR Kit
Legionella pneumophila Viability qPCR Kit
Viability PCR Crosslinker Kit
High-Concentration Viability PCR Crosslinker Kit
Prototype Maxwell® Enviro Max Kit

qPCR Kit Contents

Legionella qPCR Master Mix
20X Primer/Probe/IPC Master Mix
Legionella Quantitative DNA Standard
Neutralization Buffer
Viability PCR Crosslinker

For Research Use Only. Not for Use in Diagnostic Procedures.

U.S. Pat. Nos. 9,797,889, 9,797,890 and 10,107,800 and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582 and other patents and patents pending.

Legionella Viability qPCR Kits

Legionella qPCR Kits with Live/Dead Cell Differentiation

Detect Live Legionella with Speed and Sensitivity

How the Legionella Viability qPCR Kit Differentiates Live/Dead Cells

Sample to Results in 6 Hours

Triplex to Detect Legionella spp., Legionella pneumophila and Legionella pneumophila Serogroup 1

Available Kits

qPCR Kit Contents

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