LgBiT Expression Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 LgBiT Expression Vector sequence reference points: Base pairs 5120 CMV immediate-early enhancer/promoter 1-742 chimeric intron 857-989 T7 RNA Polymerase promoter (-17 to +3) 1033-1052 LgBiT 1065-1541 SV40 Enhancer 1883-2119 (Reverse) Sv40 Min Ori 2126-2191 Hygromycin Resistance Coding Region 2253-3290 Synthetic PolyAdenylation Signal Sequence 3314-3362 ColE1-derived plasmid replication origin 4043-4079 (Reverse) Neo-Kan Resistance 4234-5028 (Reverse) EM7 bacterial promoter 5042-5108 (Reverse) ---- 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGCT CACCATGGTC TTCACACTCG AAGATTTCGT TGGGGACTGG 1101 GAACAGACAG CCGCCTACAA CCTGGACCAA GTCCTTGAAC AGGGAGGTGT 1151 GTCCAGTTTG CTGCAGAATC TCGCCGTGTC CGTAACTCCG ATCCAAAGGA 1201 TTGTCCGGAG CGGTGAAAAT GCCCTGAAGA TCGACATCCA TGTCATCATC 1251 CCGTATGAAG GTCTGAGCGC CGACCAAATG GCCCAGATCG AAGAGGTGTT 1301 TAAGGTGGTG TACCCTGTGG ATGATCATCA CTTTAAGGTG ATCCTGCCCT 1351 ATGGCACACT GGTAATCGAC GGGGTTACGC CGAACATGCT GAACTATTTC 1401 GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA AGATCACTGT 1451 AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA 1501 CCCCCGACGG CTCCATGCTG TTCCGAGTAA CCATCAACAG TTAATCTAGA 1551 AACACCTAGG CTCTCGTACG AAGTCAGCTG ATAACGTCGA CGTATTGCGG 1601 CCGCGGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT 1651 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA 1701 TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC 1751 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCCGACGTC 1801 ACGCGTCTTG CGCAGCACCA TGGCCTGAAA TAACCTCTGA AAGAGGAACT 1851 TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA 1901 GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 1951 GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 2001 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT 2051 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG 2101 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 2151 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT 2201 TGGAGGCCTA GGCTTTTGCA AAAAGCTCGA TTCTTCTGAC ACTAGCGCCA 2251 CCATGAAGAA GCCCGAACTC ACCGCTACCA GCGTTGAAAA ATTTCTCATC 2301 GAGAAGTTCG ACAGTGTGAG CGACCTGATG CAGTTGTCGG AGGGCGAAGA 2351 GAGCCGAGCC TTCAGCTTCG ATGTCGGCGG ACGCGGCTAT GTACTGCGGG 2401 TGAATAGCTG CGCTGATGGC TTCTACAAAG ACCGCTACGT GTACCGCCAC 2451 TTCGCCAGCG CTGCACTACC CATCCCCGAA GTGTTGGACA TCGGCGAGTT 2501 CAGCGAGAGC CTGACATACT GCATCAGTAG ACGCGCCCAA GGCGTTACTC 2551 TCCAAGACCT CCCCGAAACA GAGCTGCCTG CTGTGTTACA GCCTGTCGCC 2601 GAAGCTATGG ATGCTATTGC CGCCGCCGAC CTCAGTCAAA CCAGCGGCTT 2651 CGGCCCATTC GGGCCCCAAG GCATCGGCCA GTACACAACC TGGCGGGATT 2701 TCATTTGCGC CATTGCTGAT CCCCATGTCT ACCACTGGCA GACCGTGATG 2751 GACGACACCG TGTCCGCCAG CGTAGCTCAA GCCCTGGACG AACTGATGCT 2801 GTGGGCCGAA GACTGTCCCG AGGTGCGCCA CCTCGTCCAT GCCGACTTCG 2851 GCAGCAACAA CGTCCTGACC GACAACGGCC GCATCACCGC CGTAATCGAC 2901 TGGTCCGAAG CTATGTTCGG GGACAGTCAG TACGAGGTGG CCAACATCTT 2951 CTTCTGGCGG CCCTGGCTGG CTTGCATGGA GCAGCAGACT CGCTACTTCG 3001 AGCGCCGGCA TCCCGAGCTG GCCGGCAGCC CTCGTCTGCG AGCCTACATG 3051 CTGCGCATCG GCCTGGATCA GCTCTACCAG AGCCTCGTGG ACGGCAACTT 3101 CGACGATGCT GCCTGGGCTC AAGGCCGCTG CGATGCCATC GTCCGCAGCG 3151 GGGCCGGCAC CGTCGGTCGC ACACAAATCG CTCGCCGGAG CGCAGCCGTA 3201 TGGACCGACG GCTGCGTCGA GGTGCTGGCC GACAGCGGCA ACCGCCGGCC 3251 CAGTACACGA CCGCGCGCTA AGGAGGTAGG TCGAGTTTAG ACTCTAGCCA 3301 TCACGATGGC CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG 3351 GTTTTTTGTG TGACGATTCC GGACTATAAA AGGATCTAGG TGAAGATCCT 3401 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 3451 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AAATCCTTTT 3501 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3551 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 3601 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG 3651 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3701 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 3751 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 3801 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3851 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 3901 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 3951 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4001 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 4051 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 4101 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT 4151 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 4201 CCGGTTGGGC GTCGCTTGGT CGGTCATTTC GAATCAGAAG AACTCGTCAA 4251 GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 4301 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC 4351 ACGGGTAGCC AACGCTATGT CCTGATAGCG GTCCGCCACA CCCAGCCGGC 4401 CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 4451 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCG 4501 CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT 4551 CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 4601 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC 4651 AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG 4701 CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 4751 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA 4801 AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCCTGCA 4851 GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 4901 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG 4951 TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC 5001 CTGCGTGCAA TCCATCTTGT TCAATCATGA TCTGCCTCCT GGGTTTAGTT 5051 CCTCACCTTG TCGTATTATA CTATGCCGAT ATACTATGCC GATGATTAAT 5101 TGTCAACAGT ATTAGAATTC Sequence file prepared 2/19/2020 by Feature database and feature finder version 1.0.5.0.