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E4881_Transfection-Carrier-DNA_3 E4882_Transfection-Carrier-DNA--2-x-100ug_3

Plasmid DNA Used to Dilute Expression or Reporter Vectors for Transfection

  • Maintains the total amount of DNA used in transfection while reducing the amount of expression or reporter vector
  • Lowered expression of the protein of interest can overcome potential overexpression artifacts
  • Ratio of carrier DNA to reporter DNA may need to be optimized, depending on the application
  • Supplied at a concentration of 1µg/μl

Catalog Number:

Size

Catalog Number: E4881

Consultar Precio

Catalog Number: E4882

Consultar Precio


Overview
Protocols
Specifications
Resources
Related Products

Transfection Carrier DNA is a plasmid DNA used to reduce the amount of an expression vector or reporter vector in mammalian cell transfection without reducing the overall amount of DNA. Diluting reporter DNA into Transfection Carrier DNA results in lowered expression of the protein of interest, overcoming artifacts due to overexpression. This is especially relevant when strong, constitutive promoters (e.g., CMV) are used. Depending on the application, the ratio of reporter to Transfection Carrier DNA may require optimization.

This product is made from pGEM®-3Zf(–) Vector but has been purified using a method that results in low endotoxin carryover, making it more suitable for use in transfecting mammalian cells.

Protocols

No protocols available

Specifications

Catalog Number:

Contenido

Item Part # Presentación Concentración Available Separately

Transfection Carrier DNA

E488A 5 × 20μg 1μg/μl View Product

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Contenido

Item Part # Presentación Available Separately

Transfection Carrier DNA

E488B 2 × 100μg View Product

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Resources

No related resources available