CellTox™ Green Cytotoxicity Assay
Real-Time Cell Death Assay With Multiplexing Compatibility
- Accurate cytotoxicity determination in exposures out to 72 hours
- Flexible protocol allows kinetic analysis or endpoint determination
- Multiplex with luminescent assays to obtain more data per well
Catalog Number:
Size
Catalog Number: G8741
Catalog Number: G8742
Catalog Number: G8743
Catalog Number: G8731
Monitoring Cell Death in Real Time Using the CellTox™ Green Cytotoxicity Assay
Cytotoxicity assays based on detection of biomarkers released into the media can underestimate cytotoxicity in long-term exposures of 72 hours or more because of limited stability of the biomarker detected. The CellTox™ Green Cytotoxicity Assay provides an easy, fast and accurate method to determine toxic effects during or after long-term exposure of cells in culture. CellTox Green can be combined with other methods in multiplex assays to determine mechanism of toxicity, and is easily scalable from 96- to 1536-well plate formats.
Learn how the CellTox™ Green Cytotoxicity Assay can be used to assess drug toxicity in organoids.
CellTox™ Green Dye binds DNA of cells with impaired membrane integrity.
Perform Kinetic Cytotoxicity Measures to Determine Onset of Toxicity
Dose- and exposure-dependent increases in cytotoxicity
In the experiment shown here, bortezomib caused appreciable cytotoxicity in K562 cells between the 4- and 24-hour time points. Additive fluorescence at later time points indicates continued loss of membrane integrity occurring as a function of cytotoxicity against remaining cells. The CellTox™ Green Dye was added at seeding.
Multiplex with Cell Viability Assays for More Informative Data per Well
Measure Cytotoxicity and Viability
Here, CellTox™ Green Dye was mixed with K562 Cells, which were plated, then dosed with compound. CellTiter-Glo® Cell Viability Assay Reagent was added at the end of the 72-hour exposure and luminescence (viability) measured. These two inverse measures of cell health resulted in EC50 agreement.
Determine Appropriate Timing of Caspase Assays
CellTox™ Green can be used in kinetic mode to determine the onset of cytotoxicity followed by multiplexing with the Caspase-Glo® 3/7 Assay in the same sample well to confirm caspase-3/7 activation at the appropriate time.
Differentiate Between Cytotoxic and Cytostatic Effects
In this example, K562 cells were exposed to Methotrexate, an anti-proliferative compound, for 72 hours. CellTox™ Green Dye was added to determine cytotoxicity, then CellTiter-Glo® Reagent was added to the same sample well for viability determination. A viability assay alone would have misreported the compound as cytotoxic due to less cells in the treated sample compared to the non-treated control, which continued to proliferate. Multiplexing with a cytotoxicity assay reveals no change in signal as a result of compromised membrane integrity, indicating growth inhibition and not cytotoxicity.
After several months trying to develop a robust cytotoxicity cell-based assay, we always observed high variability on the upper asymptote of the dose-response curve. Based on Promega’s recommendations, we replaced our previous cytotoxicity detection reagent by the CellTox™ Green Cytotoxicity reagent, which directly solved our issues. The cell-based assay is now fully validated with this reagent in our company.
Dr. Fabian Vandermeers, Sr R&D Technical Leader, Quality Assistance S.A.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
Lysis Solution |
G182B | 1 × 0.5ml |
|
Assay Buffer |
G610A | 1 × 10ml |
CellTox™ Green Dye, 1,000X |
G873A | 1 × 20μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
Lysis Solution |
G182B | 1 × 0.5ml |
|
Assay Buffer |
G610B | 1 × 50ml |
CellTox™ Green Dye, 1,000X |
G873A | 5 × 20μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
Lysis Solution |
G182B | 1 × 0.5ml |
|
Assay Buffer |
G610B | 2 × 50ml |
CellTox™ Green Dye, 1,000X |
G873B | 1 × 200μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
CellTox™ Green Dye, 1,000X |
G873B | 1 × 200μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Artículos
Menciones
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Elongation factor 2 kinase promotes cell survival by inhibiting protein synthesis without inducing autophagy.
2016
Cell Signal
-- -
Imaging oxygen in neural cell and tissue models by means of anionic cell-permeable phosphorescent nanoparticles.
2015
Cell Molec. Life Sci.
-- -
NAMPT inhibition sensitizes adenocarcinoma cells to tumor-selective, PAR-independent metabolic catastrophe and cell death induced by β-lapachone.
2014
Cell Death Dis.
-- -
Impact of p53 status on radiosensitization of tumor cells by MET inhibition–associated checkpoint abrogation.
2015
Mol. Cancer Res.
-- -
Brincidofovir (CMX001) inhibits BL polyomavirus replication in primary human urothelial cells.
2015
Antimicrob. Agents Chemother.
-- -
Development and validation of a luminescence-base, medium throughput assay for drug screening in Schistosoma mansoni.
2015
PLOS Negl. Trop. Dis.
-- -
In vitro ischemia decreases histone H4K16 acetylation in neural cells.
2015
FEBS Lett.
-- -
Genetic polymorphisms associated with hearing threshold shift in subjects during first encounter with occupational impulse noise.
2015
PLos ONE
-- -
Glutamine synthetase activity fuels nucleotide biosynthesis and supports growth of glutamine-restricted glioblastoma.
2015
Nat. Cell Biol.
-- -
The role of thioredoxin reductase and glutathione reductase in plumbagin-induced, reactive oxygen species-mediated apoptosis in cancer cell lines.
2015
Eur. J. Pharmacol.
-- -
New small molecules targeting apoptosis and cell viability in osteosarcoma.
2015
PLos ONE
-- -
Efficient elimination of CD103-expressing cells by anti-CD103 antibody drug conjugates in immunocompetent mice.
2015
Int. Immunopharmacol.
-- -
High USP6NL levels in breast cancer sustain chronic AKT phosphorylation and GLUT1 stability fueling aerobic glycolysis.
2018
Cancer Res.
-- -
Cytotoxicity Assays: In Vitro Methods to Measure Dead Cells.
2019
Assay Guidance Manual
-- -
APD-containing cyclolipodepsipeptides target mitochondrial function in hypoxic cancer cells.
2018
Cell Chemical Biology
-- -
Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells.
2015
Br. J. Cancer
-- -
Precision cancer medicine in the acoustic dispensing Era: Ex vivo primary cell drug sensitivity testing.
2016
J. Lab. Autom.
-- -
Topoisomerase IIα mediates TCF-dependent epithelial-mesenchymal transition in colon cancer.
2016
Oncogene
-- -
NRF2 regulates serine biosynthesis in non-small cell lung cancer.
2015
Nat. Genet.
-- -
Novel benzoxazines as inhibitors of angiogenesis.
2015
Invest. New Drugs
-- -
Real-time cell toxicity profiling of Tox21 10K compounds reveals cytotoxicity dependent toxicity pathway linkage
2017
PLos ONE
-- -
Glutamine drives glutathione synthesis and contributes to radiation sensitivity of A549 and H460 lung cancer cell lines.
2016
Biochim. Biophys. Acta
-- -
Engineering persister-specific antibiotics with synergistic antimicrobial functions.
2014
ACS Nano
-- -
Molecular mechanism for the control of eukaryotic elongation factor 2 kinase by pH: Role in cancer cell survival.
2015
Mol. Cell. Biol.
-- -
RBM45 modulates the antioxidant response in amyotrophic lateral sclerosis through interactions with KEAP1.
2015
Mol. Cell. Biol.
-- -
Oncogenic β-catenin and PIK3CA instruct network states and cancer phenotypes in intestinal organoids.
2017
J. Cell Biol.
-- -
High USP6NL Levels in Breast Cancer Sustain Chronic AKT Phosphorylation and GLUT1 Stability Fueling Aerobic Glycolysis.
2018
Cancer Res.
-- -
The nucleoside analog GS-441524 strongly inhibits feline infectious peritonitis (FIP) virus in tissue culture and experimental cat infection studies.
2018
Veterinary Microbiology
-- -
Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction.
2017
Nat. Commun.
-- -
Resveratrol-induced autophagy is dependent on IP3Rs and on cytosolic Ca2.
2017
Biochim. Biophys. Acta
-- -
Gas6-Axl signaling in presence of Sunitinib is enhanced, diversified and sustained in renal tumor cells, resulting in tumor-progressive advantages.
2017
Exp. Cell Res.
-- -
The human cancer cell active toxin Cry41Aa from Bacillus thuringiensis acts like its insecticidal counterparts.
2017
Biochem. J.
-- -
DNA damage-induced apoptosis suppressor (DDIAS), a novel target of NFATc1, is associated with cisplatin resistance in lung cancer.
2016
Biochim. Biophys. Acta
-- -
Auranofin is an apoptosis-simulating agent with in vitro and in vivo anti-leshmanial activity.
2014
ACS Chemical Biology
-- -
Analysis of toxic amyloid fibril interactions at natively derived membranes by ellipsometry.
2015
PLos ONE
-- -
Inhibition of Pseudomonas aeruginosa biofilm formation on wound dressings.
2015
Wound Repair Regen.
-- - Ver todas las menciones
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