Lumit® Second Messenger Assays
Sensitive, Simple, and Homogeneous Detection of cAMP, cGAMP and cGMP
- No Wash, Homogeneous Format: Add-and-read protocol simplifies your workflow and minimizes handling
- Exceptional Sensitivity and Specificity: Detect low-level cyclic nucleotides with minimal cross-reactivity
- Flexible and Scalable: Compatible with 96- and 384-well plates to meet varied screening demands
- Rapid, Reliable Results: Obtain luminescent readings within minutes
Catalog Number:
Choose an assay
Size
Catalog Number: CS3592A05
Catalog Number: CS3592A08
Catalog Number: CS3592A13
Overview
Lumit® Second Messenger Assays offer robust, no-wash luminescent competitive binding methods ideal for quantifying intracellular cyclic nucleotides (cAMP, cGMP or cGAMP) directly from cell lysates. Utilizing Promega's innovative Lumit® technology based on NanoLuc® Luciferase structural complementation technology, these assays deliver sensitive detection with streamlined workflows suitable for high-throughput screening and drug discovery applications.
Assay Workflow
The Lumit® cyclic nucleotide assays include a tracer composed of cyclic nucleotide labeled with SmBiT (Tracer), a primary mouse anti-cyclic nucleotide monoclonal antibody, and a secondary anti-mouse antibody conjugated to LgBiT. In the absence of unlabeled cyclic nucleotide, the tracer binds the antibodies to form a complex, bringing SmBiT and LgBiT into close proximity and reconstituting the Nanoluc® luciferase. Upon addition of Lumit® Detection Substrate, this interaction generates maximal luminescent signal output. When unlabeled cyclic nucleotide is present in increasing concentrations, it competes with the tracer for antibody binding, resulting in a reduction of luminescent signal proportional to the unlabeled cAMP concentration.
In the Literature
These peer-reviewed papers demonstrate how Lumit® Second Messenger Assays are used to monitor cAMP, cGAMP and cGMP from multiple cell types and biochemical reactions.
Lumit® cAMP Assay
Mikheil, D. et al. A bioluminescent and homogeneous assay for monitoring GPCR-mediated cAMP modulation and PDE activity. Sci. Rep. 14(1):4440.
Lumit® cGAMP Assay
Hsiao, K., et al. Homogeneous and bioluminescent biochemical and cellular assay for monitoring cGAMP and enzymes that generate and degrade cGAMP. Sci. Rep. 14, 31165.
Lumit® cGMP Assay
Murray, N.H. et al. A homogeneous bioluminescent system to monitor cyclic guanosine monophosphate. ACS Pharmacol. Transl. Sci. 6(12):1851–1858.
Protocols
No protocols available
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
2’, 3’- cGAMP Standard, 1mM |
CS3592A01 | 1 × 10μl |
Lumit® 2’, 3’-cGAMP-Tracer, 100X |
CS3592A02 | 1 × 35μl |
Anti-2’, 3’-cGAMP mAb, 100X |
CS3592A03 | 1 × 35μl |
Antibody Dilution Solution, 2X |
CS3592A04 | 2 × 1.0ml |
|
Digitonin |
G944B | 1 × 175μl |
Lumit® Detection Substrate C |
VB103A | 2 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 1 × 1.5ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102A | 1 × 30μl |
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
Anti-cAMP antibody (mouse) |
CS3592A06 | 1 × 30μl |
cAMP Tracer |
CS3592A07 | 1 × 16μl |
|
Digitonin |
G944B | 1 × 175μl |
|
cAMP, 1mM |
V642B | 1 × 100μl |
Lumit® Detection Substrate C |
VB103A | 2 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 1 × 1.5ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102A | 2 × 30μl |
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
Contenido
| Item | Part # | Presentación |
|---|---|---|
cGMP Standard, 1mM |
CS3592A09 | 1 × 10μl |
2x Neutralization Buffer |
CS3592A10 | 2 × 750μl |
cGMP-SmBiT Tracer |
CS3592A11 | 1 × 25μl |
Anti-cGMP mAb |
CS3592A12 | 1 × 25μl |
|
Digitonin |
G944B | 1 × 175μl |
Lumit® Detection Substrate C |
VB103A | 2 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 2 × 1.5ml |
Lumit® Anti-Rabbit Ab-LgBiT |
W104A | 2 × 30μl |
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
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