AMP-Glo™ Assay
Monitor AMP Concentration in Biochemical Reactions
- Use with virtually with any AMP-producing enzyme
- Well-suited for low-activity enzymes
- Sensitive to low AMP concentration
Catalog Number:
Size
Catalog Number: V5011
Catalog Number: V5012
Sensitive, Luminescent AMP Detection
The AMP-Glo™ Assay is a homogeneous assay that generates a luminescent signal from any biochemical reaction that produces AMP. It can be used to measure the activity of a broad range of enzymes, such as cyclic AMP-specific phosphodiesterases, aminoacyl-tRNA synthetases, DNA ligases and ubiquitin ligases or enzymes modulated by AMP.
The assay quantitatively monitors AMP concentration in a biochemical reaction in a wide range of plates, including high-throughput formats. The stable luminescent signal eliminates the need for an injector-equipped luminometer and allows batch-mode processing of multiple plates. The assay can be used to determine the AMP produced either in the presence or absence of ATP as a substrate.
Two reagents are supplied: one to terminate the AMP-generating enzymatic reaction, remove ATP and convert AMP produced into ADP, and a second reagent to convert ADP to ATP, which is used to generate a luminescence in a luciferase reaction. The AMP-Glo™ Assay is well suited for monitoring AMP produced in biochemical reactions catalyzed by enzymes that do not use ATP as a substrate, such as cAMP-dependent phosphodiesterases (PDE) and bacterial DNA ligases.
Screen Library Compounds for Effects on AMP-Producing Enzymes
The AMP-Glo™ Assay has a high dynamic range and produces a strong signal at low substrate conversion, allowing screening of low-activity enzymes. The assay produces minimal false hits and Z´ values greater than 0.7, and can be used to screen library compounds for effects on target enzymes such as:
- ubiquitin ligases
- cyclic AMP-specific phosphodiesterases
- aminoacyl-tRNA synthetases
- DNA ligases
- poly(A) deadenylases
- demethylases (indirect method)
AMP-Glo™ Assays contain enough reagent to perform the indicated number of reactions in a low-volume 384-well plate format using 5μl of reaction enzyme, 5μl of AMP-Glo™ I Reagent and 10μl of AMP Detection Solution. The system also can be used in standard 96-well plates (25μl:25μl:50μl).
See More Data: This peer-reviewed paper demonstrates how the AMP-Glo™ Assay was used to monitor and characterize soluble and membrane-bound ectonucleotidases CD73 and CD39.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
| AMP-Glo™ Reagent I | V503A | 1 × 5ml |
| AMP-Glo™ Reagent II | V504A | 1 × 100μl |
| Kinase-Glo® One Solution | V505A | 1 × 10ml |
| AMP, 10mM | V506A | 1 × 100μl |
|
Ultra Pure ATP, 10mM |
V915A | 1 × 500μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
| AMP-Glo™ Reagent I | V503B | 1 × 50ml |
| AMP-Glo™ Reagent II | V504B | 1 × 1ml |
| Kinase-Glo® One Solution | V505B | 1 × 100ml |
| AMP, 10mM | V506B | 1 × 1ml |
|
Ultra Pure ATP, 10mM |
V915B | 1 × 5ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
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