JM109 Competent Cells
Ready-to-Use Competent <em>E. coli</em> Cells for Recombinant Cloning and Subcloning
- Cells are ready to use—no preparation time necessary
- Transformation efficiencies guaranteed
- No worries about undesirable recombination events (recA– mutation), and no nuclease carryover in miniprep DNA (endA– mutation)
Catalog Number:
Choose usage
Size
Catalog Number: L2005
Catalog Number: L1001
Catalog Number: L2001
JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg for Cloning and Single Use, and Subcloning Efficiency at greater than 107cfu/μg.
For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.
JM109 Competent Cells are prepared according to a modified procedure of Hanahan.
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
Reference
- Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.
Protocols
Complete Protocol
Protocolos Rápidos
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Single-Use JM109 Competent Cells, >108cfu/µg |
L200B | 20 × 50μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
JM109 Competent Cells, Subcloning Efficiency |
L100A | 5 × 200μl |
|
Competent Cells Control DNA |
L107A | 1 × 30μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
|
Competent Cells Control DNA |
L107A | 1 × 30μl |
|
JM109 Competent Cells, High Efficiency |
L200A | 5 × 200μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
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