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P2151_pGEM--3Z-Vector--20ug_3

Standard Cloning Vector that can Efficiently Synthesize RNA In vitro

  • Blue/white screening to easily identify recombinant clones
  • Versatile vector used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • Multiple cloning site provides a convenient selection of restriction sites for cloning

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Overview
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The pGEM®-3Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.

The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0278VA05_4A-W

pGEM®-3Z Vector GenBank® Accession Number X65304 and vector sequence text file.

Specifications

Catalog Number:

Contenido

Item Part # Presentación

pGEM®-3Z Vector

P215A 1 × 20μg

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Resources

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