EcoRI

Cut Site:

G^▼AATT   C
C   TTAA_▲G

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Note: Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratios >25u/μg, in the absence of NaCl, in the presence of Mn²⁺, or under high pH conditions.

Incubation Conditions: Buffer H. 37°C.

Source: Escherichia coli RY 13.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

*MULTI-CORE™ Buffer is not recommended due to potential star activity.

Frequency of Cutting: