MULTI-CORE™ Buffer Pack
Convenient Aliquots of the Promega Universal Restriction Enzyme 10X Buffer
- Enables digestion of DNA with more than one enzyme in a single reaction
- Only modest adjustments in the amount of enzyme used will ensure complete multiple digestions
Catalog Number:
Size
Catalog Number: R9991
The MULTI-CORE™ Buffer Pack contains convenient aliquots of the Promega universal restriction enzyme 10X buffer. The MULTI-CORE™ Buffer is formulated to provide simple buffering conditions for performing multiple digestions. Many Promega restriction enzymes have between 50% and 100% activity in reactions using MULTI-CORE™ Buffer.
See the table of Promega 1X Restriction Enzyme Buffer Composition below.
1X Restriction Enzyme Buffer Composition.
| Buffer | pH (at 37°C) | Tris-HCl (mM) | MgCl2 (mM) | NaCl (mM) | KCl (mM) | DTT (mM) |
|---|---|---|---|---|---|---|
| A | 7.5 | 6 | 6 | 6 | — | 1 |
| B | 7.5 | 6 | 6 | 50 | — | 1 |
| C | 7.9 | 10 | 10 | 50 | — | 1 |
| D | 7.9 | 6 | 6 | 150 | — | 1 |
| E | 7.5 | 6 | 6 | 100 | — | 1 |
| F | 8.5 | 10 | 10 | 100 | — | 1 |
| G | 8.2 | 50 | 5 | — | — | — |
| H | 7.5 | 90 | 10 | 50 | — | — |
| J | 7.5 | 10 | 7 | — | 50 | 1 |
| K | 7.4 | 10 | 10 | — | 150 | 1 |
| L | 9.0 | 10 | 3 | 100 | — | — |
MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.
Notes:
- For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
- For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
- All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.
Protocols
No protocols available
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
MULTI-CORE™ 10X Buffer |
R999B | 3 × 1ml | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
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