RsaI
- Capable of digesting DNA in 15 minutes or less
Catalog Number:
Choose a concentration
Size
Catalog Number: R6371
Cut Site:
GT▼AC
CA▲TG
Note: For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
Incubation Conditions: Buffer C. 37°C.
Source: Rhodopseudomonas sphaeroides.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| 75–100% | 75–100% | 100% | <10% | <10% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 113 | 83 | 11 | 3 | 19 | 3 |
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Buffer C 10X Buffer |
R003A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
RsaI |
R637A | 1 × 1,000u |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Related Products
Usado con frecuencia junto con
Blue/Orange Loading Dye, 6X
Colorante para cargar muestras de ADN en pocillos de geles de electroforesis y seguir la migración durante esta.
G1881
Agarose, LE, Analytical Grade
Reactivo para la separación electroforética de ácidos nucleicos.
V3121, V3125
Ethidium Bromide Solution, Molecular Grade
Colorante fluorescente adecuado para la tinción de ácidos nucleicos tras electroforesis o en gradientes de cloruro de cesio.
H5041
λ DNA/EcoRI + HindIII Markers
Trece fragmentos que van desde 125-21.226pb, generados a partir de la digestión de ADN Lambda con EcoRI e HindIII.
G1731
