TaqI
- Restriction endonuclease from Thermus aquaticus YTI
Catalog Number:
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Size
Cut Site:
T▼CG A
A GC▲T
Note: Sensitive to overlapping dam methylation.
Incubation Conditions: Buffer E. 65°C. (TaqI exhibits <10% activity at 37°C.)
Source: Thermus aquaticus YTI.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | E | MULTI-CORE™ |
|---|---|---|---|---|---|
| 10–25% | 25–50% | 50–75% | 50–75% | 100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 121 | 50 | 10 | 4 | 13 | 7 |
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
TaqI |
R615A | 1 × 1,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
TaqI |
R615E | 1 × 10,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Related Products
Usado con frecuencia junto con
Blue/Orange Loading Dye, 6X
Colorante para cargar muestras de ADN en pocillos de geles de electroforesis y seguir la migración durante esta.
G1881
Agarose, LE, Analytical Grade
Reactivo para la separación electroforética de ácidos nucleicos.
V3121, V3125
Ethidium Bromide Solution, Molecular Grade
Colorante fluorescente adecuado para la tinción de ácidos nucleicos tras electroforesis o en gradientes de cloruro de cesio.
H5041
λ DNA/EcoRI + HindIII Markers
Trece fragmentos que van desde 125-21.226pb, generados a partir de la digestión de ADN Lambda con EcoRI e HindIII.
G1731
