PowerPlex® 16 HS System
Multiplex STR System for Use in DNA Typing
- All 16 loci amplified simultaneously in single tube and analyzed in single injection
- Includes all 13 CODIS STR markers, Amelogenin for gender determination and Penta D and Penta E
- Master mix more tolerant of PCR inhibitors
Catalog Number:
Size
Catalog Number: DC2101
Catalog Number: DC2100
STR Analysis Using the PowerPlex® 16 HS System
The PowerPlex® 16 HS System is a multiplex STR system for use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01, D3S1358, Penta E (labeled with fluorescein); FGA, TPOX, D8S1179, vWA and Amelogenin (labeled with TMR); CSF1PO, D16S539, D7S820, D13S317, D5S818 and Penta D (labeled with JOE). This multiplex includes all 13 CODIS STR markers, Amelogenin for gender determination and two low-stutter, highly discriminating pentanucleotide STR markers. The loci included in the PowerPlex® 16 HS System are more discriminating than competitive systems and are ideal for resolving partial matches or challenging familial cases.
The PowerPlex® 16 HS System is easy to use and includes size standard, amplification-grade water and Taq DNA polymerase already in the master mix. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. This system produces full profiles with 100pg of DNA, giving you confidence in the analysis of precious samples. The PowerPlex® 16 HS System is more tolerant of PCR inhibitors than competing STR systems and the previous version of the PowerPlex® 16 System, increasing your success rate and reducing the need for re-amplification. The PowerPlex® 16 HS System master mix is more tolerant of PCR inhibitors than competitive STR systems.
Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® ID and ID-X software and are available for download. Primer sequences, dyes and ladders are all unchanged from the PowerPlex® 16 System, providing concordance with existing databases. The PowerPlex® 16 HS System is compatible with the Spectrum Compact CE System and the Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Reference
- Konjhodic, R. et al. (ICMP) (2004) Comparative study of Penta D and Penta E with D2 and D19, IDENTITY Newsletter 5,1–2.
Decreasing amounts of template were amplified using the PowerPlex® 16 HS System. Each amplification was performed as directed in the PowerPlex® 16 HS System protocol. Once amplified, samples were separated on an Applied Biosystems 3130 Genetic Analyzer using 3kV, 5-second injection. In each case all expected alleles were called using a 50RFU threshold.
Rapid Processing Solutions for PowerPlex®
The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.
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Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
|
Internal Lane Standard 600 |
DG107A | 1 × 150μl | View Product | |
|
PowerPlex® 16 HS Allelic Ladder Mix |
DG108B | 1 × 50μl | ||
|
PowerPlex® 16 HS 10X Primer Pair Mix |
DK112B | 1 × 250μl | ||
|
PowerPlex® HS 5X Master Mix |
DP101A | 1 × 500μl | ||
|
Water, Amplification Grade |
DW099A | 5 × 1,250μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
Not For Medical Diagnostic Use.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
|
Internal Lane Standard 600 |
DG107A | 4 × 150μl | View Product | |
|
PowerPlex® 16 HS Allelic Ladder Mix |
DG108B | 4 × 50μl | ||
|
PowerPlex® 16 HS 10X Primer Pair Mix |
DK112B | 4 × 250μl | ||
|
PowerPlex® HS 5X Master Mix |
DP101A | 4 × 500μl | ||
|
Water, Amplification Grade |
DW099A | 10 × 1,250μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
Not For Medical Diagnostic Use.Condiciones de Almacenaje
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