The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. PCR products are commonly purified to remove excess nucleotides and primers.
Up to 95% recovery is achieved depending upon the DNA fragment size, and purified DNA can be eluted in as little as 15μl. This membrane-based system can bind up to 40μg of DNA, and allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation. Purified DNA routinely achieves 700 bases with >98% accuracy in automated fluorescent sequencing.
The x-tracta™ Gel Extractor tool (supplied with Cat.# A9283 and A9284) provides a convenient, safe method for removal of agarose gel fragments for further processing. The device removes a 0.13 × 0.33 inch gel piece from agarose gels for easy transfer into a microcentrifuge tube for processing.
Vacuum Adapters (Cat.# A1331) are required for use of the vacuum format with the Wizard® SV Gel and PCR Clean-Up System and must be purchased separately.