Wizard® Plus SV Minipreps DNA Purification Systems
1–20μg High-Quality Plasmid DNA from 1–10ml Cultures
- Process up to 20 minipreps in 45 minutes
- Choice of spin or vacuum purification formats
- No phenol extractions or precipitations required
Catalog Number:
Size
Additional options
Catalog Number: A1330
Catalog Number: A1460
Catalog Number: A1465
Catalog Number: A1340
Catalog Number: A1470
Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.
The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector and can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Column Wash Solution (CWA) |
A131B | 1 × 20ml | View Product |
|
Alkaline Protease Solution |
A144A | 1 × 550μl | View Product |
|
Neutralization Solution (NSB) |
A148B | 1 × 30ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711P | 1 × 20ml | View Product |
|
Cell Lysis Solution (CLA) |
A712P | 1 × 20ml | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 1 × 100ml | View Product |
|
Alkaline Protease Solution |
A144B | 1 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 1 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 1 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 1 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 4 × 250/pk | |
|
Collection Tubes |
A130B | 4 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 4 × 100ml | View Product |
|
Alkaline Protease Solution |
A144B | 4 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 4 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 4 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 4 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Column Wash Solution (CWA) |
A131B | 1 × 20ml | View Product |
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Alkaline Protease Solution |
A144A | 1 × 550μl | View Product |
|
Neutralization Solution (NSB) |
A148B | 1 × 30ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711P | 1 × 20ml | View Product |
|
Cell Lysis Solution (CLA) |
A712P | 1 × 20ml | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 1 × 100ml | View Product |
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Alkaline Protease Solution |
A144B | 1 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 1 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 1 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 1 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Otros
Related Products
Productos Similares
Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems
Métodos de purificación de plásmidos manuales o de alto rendimiento en formato de 96 pocillos.
A2250, A2255, A2258
PureYield™ Plasmid Miniprep System
Hasta 15μg de plásmido a partir de cultivos de 3ml, listo para la transfección en 10 minutos.
A1223, A1222
PureYield™ Plasmid Midiprep System
Hasta 200µg de plásmido a partir de cultivos de 50 ml, listo para la transfección en 30 minutos.
A2492, A2495, A2496
Wizard® SV Genomic DNA Purification System
Método sencillo basado en membranas, para purificar ADN a partir de células, tejidos o colas de ratón.
A2360, A2361
Usado con frecuencia junto con
Molecular Biology Lab Guide
Una guía diseñada para los científicos que acaban de empezar su carrera, con un enfoque en las tecnologías y técnicas fundamentales.
GEN1978
pGEM®-T Vector Systems
Vector T para la clonación simplificada de productos PCR.
A3600, A3610
Vac-Man® Laboratory Vacuum Manifold
Para una purificación de ácidos nucleicos rápida y eficaz. Procesa hasta 20 muestras de forma simultánea.
A7231
Column Wash Solution (CWA)
Component of several Wizard® SV DNA Purification Systems available for separate purchase.
A1311, A1318
