Flexi® Rabbit Reticulocyte Lysate System
Optimized Translation for Greater Protein Production
- Increase protein production as much as fourfold over standard lysate reactions
- For easy optimization the endogenous magnesium concentration is provided for each lot of Flexi® Lysate
- The Flexi® System contains three Amino Acid Mixtures, providing a choice of radioisotopes
Catalog Number:
Size
Catalog Number: L4540
Translation Optimization for Mg2+ and K+ Concentrations
The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg2+ and K+ concentrations and the choice to add or omit DTT. To help optimize Mg2+ for a specific message, the endogenous Mg2+ concentration of each lysate batch is stated in the product information included with this product. The Flexi® System also offers the choice of three amino acid mixtures and includes a control RNA encoding the firefly luciferase gene.
The Flexi® Rabbit Reticulocyte Lysate System, when optimized, can increase the quantity of protein produced as much as fourfold over a standard lysate reaction. The Flexi® System contains three Amino Acid Mixtures, enabling different choices of radioisotopes. Luciferase Control RNA and Luciferase Assay Reagent are included with the system as a functional control (only full-length luciferase is active).
For more information, see the Protocols & Applications Guide.
Applications:
- Optimizing translation of low-expressing messages.
- Oxidizing environment can be adjusted, which may be important for maintaining functional activity related to disulfide bridges.
- Luciferase activity can be used for the detection of inhibitory contaminants in RNA preparations.
- Investigation of translational control.
- Screening of chemical libraries for effect on translation.
Reference:
- Beckler, G.S. et al. (1995) Methods in Molecular Biology 37, 215–32.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Amino Acid Mixture Minus Cysteine |
L447C | 1 × 50μl | 1mM | |
|
Flexi® Rabbit Reticulocyte Lysate |
L455A | 5 × 200μl | ||
|
Luciferase Control RNA |
L456A | 1 × 10μl | View Product | |
|
Luciferase Assay Wells |
L457A | 1 × 3 each | ||
|
Magnesium Acetate |
L458A | 1 × 100μl | 25mM | |
|
Potassium Chloride |
L459A | 1 × 200μl | 2.5M | |
|
Luciferase Assay Reagent |
L486A | 1 × 250μl | ||
|
Amino Acid Mixture Minus Leucine |
L995A | 1 × 50μl | 1mM | |
|
Amino Acid Mixture Minus Methionine |
L996A | 1 × 50μl | 1mM | View Product |
|
DTT |
P117A | 1 × 100μl | 100mM | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
See Technical Bulletin for detailed storage information.
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