MagneHis™ Protein Purification System
Magnetic Purification of Polyhistidine- or HQ-Tagged Proteins from <i>E. coli</i> or Mammalian Cell Lysates
- No centrifugation or vacuum required after cell lysis
- Particles are compatible with a variety of common buffers
- Binding capacity up to 1mg of polyhistidine-tagged protein per 1ml of particles
Catalog Number:
Size
Catalog Number: V8500
Catalog Number: V8550
Purify Polyhistidine (His-) or HQ-Tagged Proteins
MagneHis™ Protein Purification System provides a simple, rapid, reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek® 2000 or FX or Tecan Genesis® RSP.
MagneHis™ Ni-Particles are compatible with a variety of common buffers. Binding capacity is approximately 1mg of polyhistidine-tagged protein per 1ml of MagneHis™ Ni-Particles.
Note: This product requires the use of a magnetic stand.
For more information, see the Protocols & Applications Guide.
Applications- Protein purification of polyhistidine- or HQ-tagged, expressed proteins from a crude E. coli cell lysate.
- High-throughput purification of His- or HQ-tagged proteins using automated platforms.
- Purification of His-tagged proteins from insect or mammalian cell lysates.
- Pull-down of His-tagged proteins for protein:protein interaction studies.
Purification of His-tagged methionyl tRNA synthetase.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
MagneHis™ Binding/Wash Buffer |
V851A | 1 × 50ml | |
|
MagneHis™ Elution Buffer |
V852A | 1 × 10ml | |
|
MagneHis™ Ni-Particles |
V854A | 1 × 2ml | View Product |
|
FastBreak™ Cell Lysis Reagent, 10X |
V857A | 1 × 15ml | View Product |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 7,112,552 and 7,354,750.
This product is licensed for use under U.S. Pat. No. 6,174,704.
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
MagneHis™ Binding/Wash Buffer |
V851B | 1 × 250ml | |
|
MagneHis™ Elution Buffer |
V852B | 1 × 50ml | |
|
MagneHis™ Ni-Particles |
V854B | 1 × 10ml | View Product |
|
FastBreak™ Cell Lysis Reagent, 10X |
V857B | 1 × 60ml | View Product |
|
DNase I (lyophilized) |
Z358A | 2 × 1 vial | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
U.S. Pat. Nos. 7,112,552 and 7,354,750.
This product is licensed for use under U.S. Pat. No. 6,174,704.
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