Bio-Glo-NB™ VLP Luciferase Assay Systems
Bright, Long-Lasting Luciferase Reagent for HiBiT PsVLPs
- Measure intracellular luminescence in living cells
- Optimized for elimination of extracellular background luminescence
- Simple add-mix-read protocol for rapid results
VLP Luciferase Assay Systems
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| JB1201 | Bio-Glo-NB™ VLP Luciferase Assay System | View Specifications | 25ml | Consultar Precio | |||
| JB1202 | Bio-Glo-NB™ VLP Luciferase Assay System | View Specifications | 100ml | Consultar Precio | |||
| JB1203 | Bio-Glo-NB™ VLP Luciferase Assay System | View Specifications | 10 × 100ml | Consultar Precio |
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Bio-Glo-NB™ VLP Luciferase System Assay Principle
The Bio-Glo-NB™ VLP Luciferase Assay Systems are functionally tested for performance using HiBiT-PsVLP Bioassays and intended for use with those products. When pseudotyped, HiBiT-tagged VLPs are added to target cells expressing the relevant receptors, and the presence of inhibitors or neutralizing antibodies (nAbs) prevents the entry/fusion process of the PsVLPs, resulting in HiBiT not being released intracellularly, and no luminescent signal is produced. In the absence of inhibition, the HiBiT-PsVLPs bind to the target cells and undergo membrane fusion. HiBiT is released into the target cells to bind LgBiT and generate a luminescent signal.
Standardized Reagents to Improve Your Results
Assay Workflow
The Bio-Glo-NB™ VLP Luciferase Assay is a nonlytic detection system designed to measure intracellular NanoBiT® complementation and luminescence in living cells. This system is intended for use with our quantitative mechanism-of-action (MoA)-based Reporter Bioassays. The cell impermeable Bio-Glo-NB™ DrkBiT peptide is pre-incubated with cells to prohibit extracellular NanoBiT® complementation and eliminate extracellular background. The Bio-Glo-NB™ Live-Cell Reagent contains the cell permeable substrate, furimazine, and a proprietary agent for reducing autoluminescence and decreasing assay background.
Simple Add-Mix-Read Protocol
Check out our selection of HiBiT PsVLP Bioassays.
Contact us to learn about additional PsVLP Bioassay development services.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ Live-Cell Luciferase Assay Substrate |
JB120B | 1 × 1.25ml |
Bio-Glo-NB™ Live-Cell Luciferase Assay Buffer |
JB121B | 1 × 25ml |
Bio-Glo-NB™ DrkBiT |
JB220A | 1 × 30μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ Live-Cell Luciferase Assay Substrate |
JB120B | 4 × 1.25ml |
Bio-Glo-NB™ Live-Cell Luciferase Assay Buffer |
JB121C | 2 × 50ml |
Bio-Glo-NB™ DrkBiT |
JB220A | 5 × 30μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ Live-Cell Luciferase Assay Substrate |
JB120B | 40 × 1.25ml |
Bio-Glo-NB™ Live-Cell Luciferase Assay Buffer |
JB121C | 20 × 50ml |
Bio-Glo-NB™ DrkBiT |
JB220A | 50 × 30μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Featured Resource
HiBiT-PsVLPs enable safe, rapid and quantitative assessment of virus neutralization and antibody-dependent enhancement. Non-replicating PsVLPs are suitable for BSL1/2 environments; assay run times are ≤1 day and provide a quantitative luminescence readout.
- HiBiT-PsVLPs enable safe, rapid and quantitative assessment of virus neutralization and antibody-dependent enhancement.
- Flexible HiBiT-PsVLP platform is compatible with diverse viruses.
- HiBiT-PsVLP bioassays are broadly applicable across therapeutic modalities and vaccines
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