QuantiFluor® RNA Select System
Fluorescent Quantitation of RNA with "Add-and-Read" Convenience
- Specific RNA quantitation
- Broad RNA quantitation
- Ideal for sequencing applications
- Simple to use and automation-friendly
- Ambient shipping-compatible, increased shelf life and reduced storage temperature requirements
Catalog Number:
Size
Catalog Number: E6640
Catalog Number: E6780
Simple, Specific and Accurate RNA Quantitation
The QuantiFluor® RNA Select System provides a fluorescent RNA-binding dye in an automation-friendly "add-and-read" format, simplifying RNA quantitation and speeding up your workflow. The system can accurately assess RNA concentration over a broad dynamic range (16–1,000ng/µl) and is ideal for downstream RNA sequencing applications.
Combined Sensitivity and Broad Range in a Single Dye
The QuantiFluor® RNA Select System combines high-sensitivity and broad-range quantitation in a single dye, making it ideal for RNA sequencing template inputs. Competing vendors require users to select between different dye products. The QuantiFluor® RNA Select System eliminates the confusion.
|
|
Sample |
Assay |
|
QuantiFluor® RNA Select System |
1.6ng/µl–100ng/µl* |
16–1,000ng |
|
Competitor HS Product |
0.2ng/µl–200ng/µl |
4–200ng |
|
Competitor BR Product |
0.5ng/µl–1,200ng/µl |
10–1,200ng |
*Based on 10µl sample input per assay.
Specificity to RNA
The QuantiFluor® RNA Select System specifically quantitates RNA in complex biological samples. RNA specificity ensures researchers confidently measure RNA without interference from DNA, making it ideal for applications such as RNA-seq, assessing in vitro transcription yields, Northern blot analysis, RNase protection assays, cDNA library preparation and RT-PCR.
The QuantiFluor® RNA Select System and Competitor Q System were tested for specificity to RNA. Results show comparable RNA specificity between both systems.
Add-and-Read Format
The QuantiFluor® RNA Select System is more stable than competitor dye products. The working dye solution can be mixed and stored for up to 1 week and used in an add-and-read format, greatly simplifying user workflow.
When compared for freeze-thaw stability at –20°C, the QuantiFluor® RNA Select System did not freeze, whereas the Competitor Q System did freeze. Results show superior stability of the QuantiFluor® RNA Select System over the Competitor Q System.
Sensitivity, Linearity and Dynamic Range
The QuantiFluor® RNA Select System detects as little as 1.6ng/µl RNA per assay using the Quantus™ Fluorometer (corresponding to 10µl of starting sample). The detection limit is defined as greater than three standard deviations above background RFU.
This graph shows a representative QuantiFluor® RNA Select System standard curve generated from a 1.2kb RNA transcript in a 96-well plate format. The assay linearity and dynamic range are shown for a range of 1.6ng/µl–100ng/µl (10µl in a 200µl assay).
Faster than qPCR; More Accurate than Absorbance
QuantiFluor® RNA Select System gives you results faster and at less cost than a qPCR assay, while being more accurate than absorbance methods. The system is also compatible with high-throughput formats, which means that it's just right for you.
Easy Setup on Microplate or Single-Tube Fluorometers
- System includes all the necessary reagents to quickly set up and quantitate RNA.
- Easy to set up on microplate readers or single-tube fluorometers, including Promega fluorometers (shown below).
- Also compatible with Qubit fluorometers (Qubit and Qubit Flex; not shown).
How Do Fluorescent Dyes Compare to Other Quantitation Methods?
In these short videos, Promega Applications Scientist Doug Wieczorek explains how fluorescent dye-based methods compare to other popular quantification techniques. Check them out for some quick guidelines on choosing the right nucleic acid quantitation method for your needs.
UV-Absorbance vs Fluorescence
Fluorescence vs qPCR
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
RNA Standard |
E299B | 2 × 10μg | |
QuantiFluor® RNA Select Dye |
E662A | 1 × 0.11ml | 90μM |
QuantiFluor® RNA Select Buffer |
E663A | 1 × 22ml | |
Nuclease-Free Water |
P119A-C | 1 × 1.25ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
|
RNA Standard |
E299A | 1 × 100μg | |
QuantiFluor® RNA Select Dye |
E662B | 1 × 0.55ml | 90μM |
QuantiFluor® RNA Select Buffer |
E663B | 1 × 200ml | |
Nuclease-Free Water |
P119C-C | 1 × 25ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
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