pGEM®-3Zf(–) Vector Technical Bulletin
Instructions for Use of Product(s)
P2261
Literature # TB045
The pGEM®-3Zf(-) Vector, a derivative of the pGEM®-3Z Vector, serves as a standard cloning vector and as a template for in vitro transcription. The pGEM®-3Zf(-) Vector contains SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be directly identified by color screening on indicator plates.
Summary of Changes
The following changes were made to the 2/23 revision of this document:
1. Removed JM109 cells due to discontinuation.
2. Updated Related Products.
3. Updated font and cover image.
Revised 2/23.