MagneSil® Total RNA mini-Isolation System Technical Bulletin
Instructions for Use of Product(s)
Z3351
Literature # TB328
The MagneSil® Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple purification of intact, purified total RNA from small amounts of cell culture (≤1 × 105 tissue culture cells), tissue (≤2mg tissue lysate in 100μl), or freshly isolated whole blood (≤20μl). The protocol enables manual purification using a multichannel pipettor and plate shaker or high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (≤1 × 103 cells) and freshly isolated whole blood (≤5μl) also may be performed. Total RNA purification is achieved without the need for vacuum filtration, centrifugation or precipitation. The 96-well total RNA isolation procedure takes about 30 minutes to complete on a liquid-handling workstation.
Summary of Changes
The following changes were made to the 7/25 revision of this document:
1. Updated fonts, document template and the cover image.
2. Removed discontinued products Cat.# V5291 and V8241.
3. Removed an expired patent statement.
4. The original Sections 4, 5 and 6 were removed or combined with current sections.
Revised 7/25.