P450-Glo™ Assays Technical Bulletin
Instructions for Use of Product(s)
V9001, V9002
Literature # TB325
The quick protocols linked below apply only to the P450-Glo™ CYP3A4 Assay with Luciferin-IPA substrate. Please see the P450-Glo™ Assays Technical Bulletin or P450-Glo™ Assays Protocol for information on other available P450-Glo™ Assays.
P450-Glo™ Assays provide a luminescent method to measure cytochrome P450 activity. A conventional cytochrome P450 reaction is performed by incubating the cytochrome P450 and a luminogenic cytochrome P450 substrate. The substrates of the P450-Glo™ Assays are derivatives of beetle luciferin. The derivatives are not substrates for luciferase but are converted by cytochrome P450s to luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to cytochrome P450 activity. All of the assays are biochemical assays (i.e., they may be performed using purified components or subcellular fractions). Many of these assays also can be used for cell-based CYP450 induction assays.
Summary of Change
The following change was made to the 9/16 version of this document:
Added vendor ordering information for salicylamide in Sections 10.B and 10.C.
Printed in USA. Revised 9/16.