Dual-Luciferase® Reporter Assay System Technical Manual
Instructions for Use of Product(s)
E1910, E1960, E1980
Literature # TM040
The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis, also known as sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a stabilized luminescent signal. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is simultaneously initiated by adding Stop & Glo® Reagent to the same tube. The Stop & Glo® Reagent also produces a stabilized signal from the Renilla luciferase, which decays slowly over the course of the measurement.
Summary of Change
The following change was made to the 8/23 revision of this document:
Updated Section 5.A.
Revised 8/23.