ReliaPrep™ Large Volume HT gDNA Isolation System Technical Manual

Instructions for Use of Product(s)
A2751

Literature # TM341

The ReliaPrep™ Large Volume HT gDNA Isolation System isolates genomic DNA from 1ml to 10ml of blood in a scalable format. The chemistry eliminates tedious centrifugation steps as well as the use of hazardous chemicals, which are inherent in precipitation-based chemistries. The system can be automated on a robotic liquid-handling workstation, allowing walkaway purification of genomic DNA from 1–10ml whole blood, regardless of sample storage or shipping conditions.

Summary of Changes, 11/18 Revision
The following changes were made to the 11/18 revision of this document:

1. On p. 2, the range of A₂₆₀/A₂₃₀ ratio was changed from “between 1.8 and 2.2” to “between 1.5 and 2.4” per a change in resin.
2. Section 7, Troubleshooting was updated to add possible causes and solutions for cases of low DNA purity.

Printed in USA. Revised 11/18

Complete Protocol
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