ViaScript® LgBiT mRNA Delivery System Quick Protocol
Instructions for Use of Product(s)
NE1120, NE1130, NE1140
Literature # FB262
Proteins fused to the 11-amino-acid HiBiT tag (e.g., via CRISPR-mediated gene editing) can be kinetically monitored in live cells expressing LgBiT protein, which binds with high affinity to HiBiT to reconstitute NanoBiT® luciferase. The ViaScript® LgBiT mRNA Delivery System enables uniform, high-efficiency transfection of LgBiT mRNA, facilitating live-cell kinetic assays to monitor changes in HiBiT-tagged protein levels.
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