Cholesterol/Cholesterol Ester-Glo™ Assay Technical Manual
Instructions for Use of Product(s)
J3190, J3191
Literature # TM601
The Cholesterol/Cholesterol Ester-Glo™ Assay provides a luminescent method for measuring cholesterol and cholesterol esters in cultured cell lysates and other biological samples such as lipoprotein fractions, cell culture medium, serum and tissue homogenates. Cholesterol is an essential lipid involved in steroidogenesis, bile acid synthesis, cell signaling and maintenance of membrane structure. However, excessive levels of cholesterol contribute to inflammation, atherosclerosis, metabolic disease and cancer. In fact, the cholesterol content of lipoproteins is routinely used as an indicator of cardiovascular risk.
The Cholesterol/Cholesterol Ester-Glo™ Assay measures cholesterol using a cholesterol dehydrogenase that links the presence of cholesterol to the production of NADH and the activation of a proluciferin that produces light with luciferase. For measuring Cholesterol Ester, the assay includes a cholesterol esterase that will remove the fatty acid from cholesterol esters to produce one molecule of cholesterol per molecule of ester. The amount of cholesterol esters is determined from the difference of cholesterol measured in the absence (free cholesterol) and presence (total cholesterol) of esterase.
Summary of Changes
The following changes were made to the 6/23 revision of this document:
1. The legend for Figure 3 was updated.
2. Equations were reformatted and miscellaneous text edits made.
3. The font was updated.
Revised 6/23.