GDP-Glo™ Glycosyltransferase Assay Technical Manual
Instructions for Use of Product(s)
VA1090, VA1091, VA1092
Literature # TM505
The GDP-Glo™ Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use GDP-sugars as donor substrates and release GDP as a product. Once the glycosyltransferase reaction is complete, an equal volume of GDP Detection Reagent is added to simultaneously convert the GDP product to ATP and generate light in a luciferase reaction. The light generated is detected using a luminometer. Luminescence can be correlated to GDP concentration by using a GDP standard curve. The light output is proportional to the concentration of GDP from low nM to 25μM.
Summary of Changes
The following changes were made to the 7/25 revision of this document:
1. Updated the cover image and fonts.
2. Updated the patent statement.
3. Updated numerical values in and around Table 2, Section 4.C.
4. Added a new reference 5, changed existing reference 5 to 6.
5. Changed the serial dilution of FUT7 enzyme in Section 4.B from 0–500ng/reaction to 0–100ng/reaction.
6. Made miscellaneous text edits.
Revised 7/25.