IL-12 Bioassay Technical Manual
Instructions for Use of Product(s)
JA2601, JA2605, JA3601, JA3605
Literature # TM608
The IL-12 Bioassay is a bioluminescent cell-based assay designed to measure IL-12 stimulation or inhibition. The IL-12 Bioassay Cells are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. The IL-12 Bioassay consists of human cells engineered to express the IL-12 receptor and a luciferase reporter driven by a response element (RE). When IL-12 binds to IL-12R, it transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using the Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following change was made to the 3/20 revision of TM608:
In Section 2. Product Components and Storage Conditions, the amount of RPMI 1640 Medium was changed to 36ml.
Printed in USA. Revised 3/20.