Lumit™ FcRn Binding Immunoassay Technical Manual
Instructions for Use of Product(s)
W1151, W1152
Literature # TM611
The Lumit™ FcRn Binding Immunoassay is a novel homogeneous (no-wash) competition assay, based on NanoBiT® technology, to measure the interaction between human FcRn and Fc proteins, including antibodies. NanoLuc® Binary Technology (NanoBiT) is a structural complementation reporter designed for biomolecular interaction studies. The NanoBiT® system is composed of two subunits, the 18kDa Large BiT (LgBiT) and the 11 amino acid peptide Small BiT (SmBiT) that have been optimized for stability and minimal self-association.
In the Lumit™ FcRn Binding Immunoassay, a human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. A C-terminal biotinylated human FcRn bound to streptavidin-SmBiT (hFcRn-SmBiT) is used as the target. In the absence of an antibody analyte, Tracer-LgBiT binds to the hFcRn-SmBiT target, resulting in maximum luminescence signal. In samples containing analyte, nonlabeled IgG will compete with Tracer-LgBiT for binding to the FcRn target, resulting in a concentration-dependent decrease in the luminescent signal.
Summary of Changes
The following changes were made to the 7/22 revision of this document:
- In Section 4, Materials to Be Supplied by the User, “polypropylene” was added to the description of multiwell plates.
- Font was updated.
Revised 7/22