Lumit® Human IL-1β Immunoassay Technical Manual
Instructions for Use of Product(s)
W6010, W6011, W6012
Literature # TM645
The Lumit® Human IL-1β Immunoassay is a homogeneous, bioluminescent assay for detecting interleukin-1β (IL-1β) released from cells without the need for sample transfers or wash steps. This Lumit® IL-1β Immunoassay is based on NanoLuc® Binary Technology (NanoBiT®), a luminescent structural complementation system designed for biomolecular interaction studies. The system is composed of two subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that have been optimized for stability and minimal spontaneous association. In this assay, a sample is incubated with a pair of anti-human IL-1β monoclonal antibodies covalently labeled with SmBiT or LgBiT. When the labeled antibodies recognize and bind to released IL-1β, the complementary LgBiTs and SmBiTs are brought into proximity, reconstituting NanoBiT® enzyme and generating luminescence in the presence of the Lumit® substrate. Luminescence generated is directly proportional to the amount of analyte present in the sample.
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Lumit was updated to a registered trademark.
2. Lumit® Human IL-1β Standard (Cat.# W116A-C) was added to Sections 4.B, 5.B and 9.D.
3. Lumit® Mouse IL-1β Standard (Cat.# W119A-C) was added to Section 9.D.
4. Added a third party trademark attribution.
Revised 5/25.