Nano-Glo® Endurazine™ and Vivazine® Live Cell Substrates Technical Manual
Instructions for Use of Product(s)
N2570, N2571, N2572, N2580, N2581, N2582, N2590
Literature # TM550
The Nano-Glo® Endurazine™ and Vivazine® Live Cell Substrates are versions of furimazine that enable live-cell, nonlytic assays for periods lasting several hours or days. For both substrates, a slow rate of ester hydrolysis leads to the steady release of furimazine throughout the experiment, a process catalyzed by cellular esterases. Once formed, furimazine can serve as a substrate for NanoLuc® or NanoBiT® luciferases. NanoBiT® luciferase consists of Large BiT (LgBiT) bound to Small BiT (SmBiT) or HiBiT peptides. Both substrates contain a proprietary compound that reduces furimazine autoluminescence.
Summary of Changes
The following changes were made to the 10/25 revision of this document:
1. Updated Vivazine to a registered trademark.
2. Replaced the cover page and document font.
3. Updated the patent statement.
4. Made minor text edits.
Revised 10/25.