RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay Technical Manual
Instructions for Use of Product(s)
JA1000, JA1001, JA1011, JA1012
Literature # TM507
The RealTime-Glo™ Apoptosis and Necrosis Assay measures exposure of phosphatidylserine (PS) on the outer leaflet of the cell membrane during the apoptotic process, and loss of membrane integrity during secondary necrosis. Annexin V binding is detected with a simple luminescence signal, and necrosis is detected with a fluorescent DNA binding dye.
The add-and-read method monitors the progression of apoptosis in real time with a simple method that does not require wash steps or flow cytometry. The plate-based assay allows repeated measurements from the same sample well. A multimode plate-reader is required for detection of luminescence and fluorescence signals.
Summary of Changes
The following changes were made to the 10/25 revision of this document:
1. Updated the cover image and fonts.
2. Removed discontinued products from Section 6.D, Related Products.
3. Removed two expired patent statements and added a patent statement.
4. Changed Notes from numerical to alphabetical lists.
5. Made minor text edits and corrected a typographical error.
Revised 10/25.