RealTime-Glo™ Extracellular ATP Assay Technical Manual
Instructions for Use of Product(s)
GA5010, GA5011, GA5012
Literature # TM652
The RealTime-Glo™ Extracellular ATP Assay uses an optimized, bioluminescence detection chemistry to measure ATP that has been released into the cultured cell environment as a result of cell death, stress or activation. The nonlytic, homogeneous reagent is created by rehydrating the assay substrate with your preferred growth medium and applied directly to cells at the time of dosing or treatment to assess continuous ATP release during stimulus exposures of up to 24 hours. Longer exposure times can be addressed using a staggered reagent addition protocol. The reagent is well-tolerated by a wide variety of both suspension and attachment-dependent cell types during standard experimental exposures and does not adversely affect cell health when used at the recommended concentrations. Additionally, the reagent can be used to measure total ATP at an exposure endpoint to assess the relative health of the cell population.
Summary of Changes
The following changes were made to the 5/24 revision of this document:
1. Updated protocol in Section 3.C.
2. Made general text edits
3. Updated font.
Revised 5/24.