PowerPlex® 4C Matrix Standard Technical Manual
Instructions for Use of Product(s)
DG4800
Literature # TMD048
Proper generation of a spectral calibration file is critical to evaluate multicolor systems with the Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. The PowerPlex® 4C Matrix Standard consists of DNA fragments labeled with four different fluorescent dyes (fluorescein, JOE, TMR and CXR) in one tube. The spectral calibration is performed using the F dye set. Once, generated, the spectral calibration file is applied during sample detection to calculate the spectral overlap between the four different dye colors and separate the raw fluorescent signals into individual dye signals. The PowerPlex® 4C Matrix Standard can be used with any of the 4-dye Promega STR amplification systems.
The PowerPlex® 4C Matrix Standard was developed for use with the Promega 4-color PowerPlex® Systems and is compatible with the Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Summary of Changes
The following changes were made to the 12/21 revision of this document:
1. Updated Sections 3.B, 4 and 5.B.
2. Removed Related Products section.
3. Updated cover page.
Revised 12/21.