PowerPlex® 5C Matrix Standard Technical Manual
Instructions for Use of Product(s)
DG4850
Literature # TMD049
Proper generation of a spectral calibration file is critical to evaluate multicolor STR data on multicapillary electrophoresis instruments. The PowerPlex® 5C Matrix Standard consists of DNA fragments labeled with five different fluorescent dyes (fluorescein, JOE, TMR-ET, CXR-ET and WEN) in one tube. The spectral calibration is performed using the G5 dye set. Once generated, the spectral calibration fi le is applied during sample detection to calculate the spectral overlap and separate the raw fluorescent signals into individual dye signals. The PowerPlex® 5C Matrix Standard can be used with any of the 5-dye Promega STR amplification systems.
The PowerPlex® 5C Matrix Standard was developed for use with the Promega 5-color PowerPlex® Systems and is compatible with the Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Summary of Changes
The following changes were made to the 10/23 revision of this document:
1. Removed any mention of ABI PRISM® Genetic Analyzers.
2. Updated Section 7.
3. Made minor text edits.
4. Updated the cover page and replaced document font.
Revised 10/23.