PowerPlex® CS7 System Technical Manual
Instructions for Use of Product(s)
DC6613
Literature # TMD042
The PowerPlex® CS7 System allows co-amplification and three-color detection of seven STR loci, including LPL, F13B, FESFPS, F13A01, Penta D, Penta C and Penta E. One primer for each of the LPL, F13B, PESFPS, F13A01, and Penta D loci is labeled with fluorescein (FL); one primer for the Penta E locus is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for the Penta C locus is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All seven loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® CS7 System contains two loci, Penta D and Penta E, which overlap with the loci included in the PowerPlex® 16 HS System. This feature allows the PowerPlex® CS7 System to be used as a confirmatory kit in paternity applications using the five unshared STR loci to supplement the genotype and increase the available information.
Summary of Changes
The following changes were made to the 7/22 revision of this document:
1. Removed mention of ABI PRISM® Genetic Analyzers.
2. Added ProFlex® PCR System and removed GeneAmp® PCR System 9600 thermal cycler.
3. Updated Section 6.J.
4. Removed original Section 9.B.
5. Revised Section 9.D.
6. Removed disclaimers.
7. Incorporated other general updates.
8. Moved to a new template.
Revised 7/22.