PowerPlex® Matrix Standards for Use on the Spectrum CE System Technical Manual
Instructions for Use of Product(s)
DG4850, DG4900, DG5010
Literature # TMD068
Proper generation of spatial and spectral calibration files is critical to evaluate multicolor systems with the Spectrum CE System. The PowerPlex® Matrix Standards consist of DNA fragments labeled with different fluorescent dyes. Matrix standards are used to create spatial and spectral calibrations in a single calibration assay for each dye set. Fragments of the matrix standard are first used to create a spatial calibration of the array, then a spectral calibration of the dyes. Once generated, the spectral calibration file is applied during sample detection to calculate the spectral overlap and separate the raw fluorescent signals into individual color signals.
Summary of Change
The following change was made to the 12/24 revision of this document:
1. Replaced the instrument photo on the cover page.
Revised 12/24.