HaloTag™ Interchangeable Labeling Technology for Cell Imaging Protein Capture and Immobilization
1Promega Corporation, 2Promega Biosciences, Incorporated
Publication Date: 2005
Abstract
The HaloTag™ Interchangeable Labeling Technology is designed to provide new options for rapid, site-specific labeling of proteins in living cells and in vitro. The technology is based on the efficient formation of a covalent bond between the HaloTag™ Protein and synthetic ligands. The covalent bond forms rapidly under physiological conditions, is highly specific and essentially irreversible, yielding a stable complex even under denaturing conditions. The HaloTag™ Ligand can carry a variety of functionalities, including fluorescent labels, affinity handles and attachments to a solid phase. The flexibility to create labeled HaloTag™ fusion proteins with a wide range of optical properties and functions will allow researchers to image and localize labeled HaloTag™ Protein fusions in live- or fixed-cell populations as well as isolate and analyze HaloTag™ Protein fusions and protein complexes.
Promega Notes 89, 2–6.
