Lumit Immunoassays for Monitoring PROTAC and Molecular Glue Interactions and Ternary Complex Formation
Part # PS448
Abstract
Proteolysis-targeting chimeras (PROTACs) are heterobifunctional small molecules designed to degrade a specific target protein. PROTACs have great therapeutic promise as they offer a new mechanism of action for drug development. To continue the rapid growth in the field of PROTACs, high-throughput screening techniques are required to discover new PROTACs targeting different proteins and E3 ligases.
We present Lumit™ Protein Interaction Immunoassay, a homogenous (no-wash) bioluminescent assay to measure protein-protein or protein-small molecule interactions in solution. Using streptavidin and antibodies that recognize commonly used protein tags coupled with NanoLuc® Technology, Lumit™ assays have a simple add-and-read protocol suitable for multiplexing and high-throughput screens.
Here, we demonstrate how Lumit™ Immunoassays can be used to monitor both binary interactions and ternary PROTAC-mediated complex formation.