BCIP/NBT Color Development Substrate
(5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium)
- Quality tested and qualified for use in blotting
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Size
BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT.
Preparation of Substrates to Detect Alkaline Phosphatase: For every 5ml of alkaline phosphatase buffer (100mM Tris-HCl [pH 9.0], 150mM NaCl, 1mM MgCl2), add 33μl NBT and 16.5μl BCIP. Add the NBT first, mix, add the BCIP, and mix again. Use within 1 hour, and discard any unused solution.
Concentration: BCIP (50mg/ml) in 100% dimethylformamide; NBT (50mg/ml) in 70% dimethylformamide.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
NBT |
S380C | 1 × 2.5ml |
|
BCIP |
S381C | 1 × 1.25ml |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
Resources
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