SYK Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The SYK Kinase Enzyme System contains:

• SYK Kinase, 10μg (Human, recombinant full-length). MW: ~100kDa.
• Poly (4:1 Glu, Tyr) Peptide Substrate.
• Reaction Buffer, DTT.

Recombinant full-length human SYK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells. It is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis. In B cells, SYK plays a crucial role in intracellular signal transduction induced by oxidative stress as well as antigen receptor engagement. SYK has been shown to act as a potential tumor suppressor in breast cancer. Absence of SYK protein in primary breast tumors is correlated with poor outcomes. SYK-deficient cells have increased motility that is restored to normalcy by replacement with wild-type SYK.

SYK NCBI Database Entry.

The SYK Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.