HB101 Competent Cells
Ready-to-Use Competent <em>E. coli</em> Cells for Recombinant Cloning and Subcloning
- Cells are ready to use—no preparation time necessary
- Transformation efficiencies guaranteed
- No worries about undesirable recombination events (recA– mutation)
Catalog Number:
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Size
Catalog Number: L2015
Catalog Number: L2011
HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.
For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.
HB101 Competent Cells are prepared according to a modified procedure of Hanahan.
HB101 Genotype: F–, thi-1, hsdS20 (rB–, mB–), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.
Reference
- Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Single-Use HB101 Competent Cells, >108cfu/µg |
L201B | 20 × 50μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Competent Cells Control DNA |
L107A | 1 × 30μl |
|
HB101 Competent Cells, High Efficiency |
L201A | 5 × 200μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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