LigaFastâ„¢ Rapid DNA Ligation System
Ligate DNA Inserts into Plasmid Vectors in 15 Minutes or Less at Room Temperature
- DNA inserts with blunt ends or 5´ or 3´ overhangs can be used for ligation
- No purification of ligated DNA needed prior to heat-shock transformation in E. coli
- Ligations performed using the LigaFastâ„¢ System are comparable to standard overnight ligations
Catalog Number:
Size
Catalog Number: M8221
Catalog Number: M8225
The LigaFastâ„¢ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFastâ„¢ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.
The LigaFastâ„¢ System is Blue/White Cloning Qualified, providing a higher level of quality control for enzymes used in cloning applications.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.
QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.
Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
2X Rapid Ligation Buffer |
C671A | 1 × 200μl | View Product |
|
T4 DNA Ligase |
M180A | 1 × 100u |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
2X Rapid Ligation Buffer |
C671B | 2 × 500μl | View Product |
|
T4 DNA Ligase |
M180B | 1 × 500u |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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