pGEM®-3Zf(+/–) Vectors
Standard Cloning Vectors That Can Produce ssDNA and In vitro Transcribe Sense and Antisense RNA
- Blue/white screening for easy identification of recombinant clones
- In vitro transcription using the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
- The difference between + and – vectors is orientation of the f1 origin
Catalog Number:
Choose a vector
Size
Catalog Number: P2271
Catalog Number: P2261
The pGEM®-3Zf(+) and pGEM®-3Zf(–) Vectors contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM®-3Zf(+) and -3Zf(–) Vectors can be used as standard cloning vectors and as templates for in vitro transcription.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
pGEM®-3Zf(+) Vector GenBank® Accession Number X65306 and vector sequence text file.
pGEM®-3Zf(–) Vector GenBank® Accession Number X65307 and vector sequence text file.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pGEM®-3Zf(+) Vector |
P227A | 1 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pGEM®-3Zf(–) Vector |
P226A | 1 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for storage recommendations.
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Frequently Used With
JM109 Competent Cells
E. coli strain ready for transformation; offers blue/white selection.
L2005, L1001, L2001
RiboMAX™ Large-Scale RNA Production Systems
Compatible with modified or unmodified nucleotides. Produces 2–5mg/ml RNA.
P1280, P1300
Riboprobe® Systems
Generate high-specific-activity RNA probes or transcripts; choice of SP6, T7 or T3 systems.
P1420, P1430, P1440
Riboprobe® Combination Systems
Generate high-specific-activity RNA probes or transcripts; choice of SP6/T7 or T3/T7 systems.
P1450, P1460
