BamHI
- Capable of digesting DNA in 15 minutes or less
- Active and capable of digestion directly in GoTaq® Green Master Mix
- Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6021
Cut Site:
Gâ–¼GATCÂ C
CÂ Â CTAGâ–²G
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Usage Notes:
- Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn2+.
- Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.
Incubation Conditions: Buffer E. 37°C.
Source: Bacillus amyloliquefaciens H.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | E | MULTI-COREâ„¢ |
|---|---|---|---|---|---|
| 75–100%* | 75–100% | 75–100% | 50–75% | 100% | 75–100% |
*Buffer A is not recommended due to potential star activity.
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 5 | 3 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
BamHI |
R602A | 1 × 2,500u | ||
|
MULTI-COREâ„¢ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
