NdeI
- Capable of digesting DNA in 15 minutes or less
- Active and capable of digestion directly in GoTaq® Green Master Mix
Catalog Number:
Choose a concentration
Size
Catalog Number: R6801
Cut Site:
CAâ–¼TAÂ TG
GTÂ Â ATâ–²AC
Notes:
- Star activity may be observed with glycerol concentrations >10%.
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Ends generated with NdeI can be directly ligated to ends generated by VspI. Neither recognition site is regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Neisseria denitrificans.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-COREâ„¢ |
|---|---|---|---|---|
| <10% | <10% | 25–50% | 100% | 25–50% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 7 | 2 | 0 | 1 | 3 | 1 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer D 10X Buffer |
R004A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
NdeI |
R680A | 1 × 500u | ||
|
MULTI-COREâ„¢ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
Related Products
Frequently Used With
LigaFastâ„¢ Rapid DNA Ligation System
Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.
M8221, M8225
Blue/Orange Loading Dye, 6X
Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.
G1881
Agarose, LE, Analytical Grade
Reagent for electrophoretic separation of nucleic acids.
V3121, V3125
Ethidium Bromide Solution, Molecular Grade
Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.
H5041
