Maxwell® RSC Genomic DNA Kit

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Consistent and Reliable Automated Genomic DNA Extraction from a Broad Range of Human Sample Types for a Variety of Downstream Assays

  • Extracts high-quality DNA from whole blood, buffy coat, bone marrow, buccal swabs, cells and tissues
  • Processes different sample types simultaneously in a single run of the Maxwell® RSC Instrument
  • Provides high-performance DNA extraction for amplification-based assays
  • Batch multiple sample types for workflow flexibility with the same yield and purity of single-sample-type kits for sensitive downstream applications

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Catalog number selected: AS1880

$ 405.00
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Maxwell® RSC Genomic DNA Kit
48 preps
$ 405.00
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High-Efficiency, Automated Genomic DNA Extraction from a Broad Range of Sample Types

The Maxwell® RSC Genomic DNA Kit is used in combination with the Maxwell® RSC or RSC 48 Instrument to purify genomic DNA from multiple different human sample types in a single instrument run. Compatible sample types include human whole blood, buffy coat, bone marrow, buccal swabs, tissues, and cells isolated from tissue cultures or various biological fluids such as urine and amniotic fluid. The Maxwell® RSC Genomic DNA Kit offers research laboratories a platform solution with built-in flexibility for consistent and reliable DNA extraction from different sample types and input amounts for use in a variety of sensitive downstream applications.

14271CB - Maxwell RSC Instruments
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Merche Bermejo Villodre, Laboratory Manager – Valencia healthincode

The kit gives us a very good yield and quality of DNA from different kinds of samples compared to other kits that we have used. The most interesting feature is that we get good results even from samples that are originally expected to have low amounts of DNA and we are able to use one kit, for all samples at the same time.

Merche Bermejo Villodre, Laboratory Manager – Health In Code, Valencia

Flexible Workflow Allows Multiple Sample Types to be Processed in a Single Extraction Run

The Maxwell® RSC Genomic DNA Kit contains all the necessary reagents for DNA extraction from different sample types. All sample types use the same Maxwell® RSC Instrument method (~40 minutes) following a sample-specific lysis step. The Maxwell® RSC Genomic DNA Kit allows different sample input amounts, and the purified DNA can be eluted in 50–200µl of Elution Buffer.

Sample Types Compatible with the Maxwell® RSC Genomic DNA Kit

Maxwell CSC Genomic DNA Kit purifies DNA from whole blood, buffy coat or bone marrow samples
Maxwell CSC Genomic DNA Kit purifies DNA from buccal swabs, cells or tissue.
Sample-specific lysate preparation

Simultaneous DNA Extraction on Maxwell® RSC Instruments

Schematic of sample addition and sample mixing in the Maxwell cartridge.
Schematic of particle capture and nucleic acid binding steps in the Maxwell DNA purification method.
Schematic of washing and elution steps in the Maxwell DNA purification method.

Maxwell® RSC Genomic DNA Kit Extracts High-Quality DNA from Different Human Sample Types

Genomic DNA yields of whole blood with 4 different anticoagulants.
DNA extraction sample quality chart of absorbance-based purity ratios.
Concentration of DNA samples from Panel B.

The Maxwell® RSC Genomic DNA Kit used with the Maxwell® RSC or the Maxwell® RSC 48 Instrument consistently extracts high yield and quality of DNA from multiple sample types. Panel A. Genomic DNA was extracted from 300µl of whole blood (n=12) with different anticoagulants and eluted in either 50µl or 200µl of Elution Buffer. Panel B. Average purity ratios of DNA extracted from different sample types as determined by absorbance. Panel C. DNA was extracted from 300µl each of buffy coat and bone marrow, 5 × 106 HEK293 cells, 50mg of pancreatic tissue, and one or two buccal swabs. All samples were eluted in 200µl of Elution Buffer, and the concentration and quality of samples was determined by absorbance.

Reliable Amplification using Genomic DNA Extracted from Different Human Sample Types using the Maxwell® RSC Genomic DNA Kit

DNA extracted using the Maxwell® CSC Genomic DNA Kit shows consistent amplification in qPCR.

DNA extracted from multiple sample types using the Maxwell® RSC Genomic DNA Kit shows consistent amplification in a qPCR assay. DNA was extracted from whole blood, buffy coat, bone marrow, HEK293 cells, pancreatic tissue and one or two buccal swabs (n=12). The DNA was quantified by absorbance, diluted to a concentration within the qPCR standard curve and used as a template to amplify a 300-base-pair target in a qPCR assay.

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

Item Part # Size Available Separately

Elution Buffer

 A828D 1 × 20ml View Product

Maxwell® RSC Cartridge (RSCB)

 AS140A 48 × 1 each

Elution Tubes (0.5ml)

 AS620A 1 × 50/pk View Product

Lysis Buffer

 MC110A 1 × 20ml

Proteinase K (PK) Solution

 MC111A 2 × 1ml

RNase A Solution

 MC120A 1 × 1ml

Lytic Enhancer (LE2)

 MC153A 1 × 20ml

RSC Plungers

 AS1670 1 × 48/pack View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

Software and Firmware

Other Resources

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Frequently Used With

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Maxwell® RSC 48 Instrument

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Service and warranty products for the Maxwell® RSC 48 Instrument.

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SA1347, SA1348, SA1349

Maxwell® RSC 48 Qualification Products

Installation Qualification (IQ) and Operational Qualification (OQ) products for the Maxwell® RSC 48 Instrument.

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Maxprep® Liquid Handler with Maxwell® Instrument Carriers

Automated sample preprocessing and post-extraction sample preparation for quantitation, normalization and amplification setup.

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