PolyATtract® mRNA Isolation Systems
Isolates Highly Pure mRNA from Total RNA
- Protocol complete in approximately 45 minutes
- Isolated mRNA is suitable for use in in vitro translation, RT-PCR and cDNA synthesis
- Cat.# Z5200 and Z5210 contain sufficient reagents for 3 separate mRNA isolations, each from 1-5mg total RNA
- Cat.# Z5300 and Z5310 contain sufficient reagents for 15 separate mRNA isolations, each from 100-1,000µg total RNA
Catalog Number:
Size
Add a magnetic stand
Catalog Number: Z5210
Catalog Number: Z5200
Catalog Number: Z5300
Catalog Number: Z5310
The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The highly pure mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P119C | 3 × 25ml | View Product |
|
SSC, 20X |
Z525A | 3 × 1.4ml | |
|
Biotinylated Oligo(dT) Probe |
Z526A | 1 × 35μl | View Product |
|
mRNA User Tube |
Z528A | 3 × 1 each | |
|
Streptavidin MagneSphere® Paramagnetic Particles |
Z548F | 3 × 3ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P119C | 3 × 25ml | View Product |
|
SSC, 20X |
Z525A | 3 × 1.4ml | |
|
Biotinylated Oligo(dT) Probe |
Z526A | 1 × 35μl | View Product |
|
mRNA User Tube |
Z528A | 3 × 1 each | |
|
MagneSphere® Technology Magnetic Separation Stand (two-position) |
Z5333 | 1 × 12 × 75mm | |
|
Streptavidin MagneSphere® Paramagnetic Particles |
Z548F | 3 × 3ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
|
SSC, 20X |
Z525A | 2 × 1.4ml | |
|
Biotinylated Oligo(dT) Probe |
Z526B | 1 × 50μl | View Product |
|
MagneSphere® Technology Magnetic Separation Stand (two-position) |
Z5332 | 1 × 1.5ml | |
|
Streptavidin MagneSphere® Paramagnetic Particles |
Z548C | 15 × 0.6ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
|
SSC, 20X |
Z525A | 2 × 1.4ml | |
|
Biotinylated Oligo(dT) Probe |
Z526B | 1 × 50μl | View Product |
|
Streptavidin MagneSphere® Paramagnetic Particles |
Z548C | 15 × 0.6ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
No related resources available
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Strong rare earth magnets for magnetic separation applications.
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